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Wednesday, May 13, 2020 | History

5 edition of Gene Amplification and Analysis, Volume 2 found in the catalog.

Gene Amplification and Analysis, Volume 2

Structural Analysis of Nucleic Acids

by Jack G. Chirikjian

  • 345 Want to read
  • 15 Currently reading

Published by Elsevier Science Ltd .
Written in English


The Physical Object
Number of Pages616
ID Numbers
Open LibraryOL7529411M
ISBN 100444006362
ISBN 109780444006363

The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. Get this from a library! Whole genome amplification: methods and protocols. [Thomas Kroneis;] -- This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers.

  Androgen receptor (AR)-gene amplification, found in 20–30% of castration-resistant prostate cancer (CRPCa) is proposed to develop as a consequence of hormone-deprivation therapy and be a prime cause of treatment failure. Gene Amplification Analyses. Genomic DNA was extracted from 30 m section of formalin fixed, paraffin embedded (FFPE) tumor tissue, in a GenoMTM Robotic Workstation (GenoVision, Oslo, Norway), as described by the manufacturer (GenoMTM, Qiagen protocol: Isolation of genomic DNA from paraffin-embedded sections using the MagAttract DNA Mini M48 Kit, December , GenoVision).

Gene amplification. From Wikipedia, the free encyclopedia. (Redirected from DNA amplification) Jump to navigation Jump to search. Gene amplification refers to a number of natural and artificial processes by which the number of copies of a gene is increased "without a proportional increase in other genes".   The study of clinical samples is often limited by the amount of material available to study. While proteins cannot be multiplied in their natural form, DNA and RNA can be amplified from small specimens and used for high-throughput analyses. Therefore, genetic studies offer the best opportunity to screen for novel insights of human pathology when little material is available. Precise estimates.


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Gene Amplification and Analysis, Volume 2 by Jack G. Chirikjian Download PDF EPUB FB2

: Gene Amplification and Analysis, Volume 2: Structural Analysis of Nucleic Acids (): Jack G. Chirikjian, Takis S. Papas: Books. Gene Amplification in Mammalian Cells: A Comprehensive Guide 1st Edition by Rodney E. Kellems (Author) ISBN ISBN Why is ISBN important. ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book.

Cited by: The human ets-1 gene was found to translocate from chromosome 11 to 4 in t(4;11)(q21;q23) and the human ets-2 gene was found to translocate from chromosome 21 to 8 in t(8;21)(q22;q22).

Abstract. We performed a correlative study to assess HER-2/neu oncogene amplification and protein overexpression. Gene amplification was determined by simultaneous hybridization of human breast tumor DNA with probes for the oncogene and a single copy reference gene, lmmunohistochemistry with a murine anti–HER-2/neu antibody was done on different portions of the Cited by: 1.

Gene Amplification and Analysis Vol. 5, Restriction Endonucleases and Methylases Edited by Jack G. Chirijian Elsevier Biomedical Press; Amsterdam, New York, x + pages. $, Dfl This is the second volume in this series to be devoted to restriction endonucleases.

Other volumes include one on structural analysis of. Page - Frohman, MA, Dush, MK, and Martin, GR () Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Proc. Natl. Acad. Sci. Edwards, JBDM, Delort, J., and Mallet, J. () Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5' ends of mRNAs and for /5(16). USB2 US09/, USA USB2 US B2 US B2 US B2 US A US A US A US B2 US B2 US B2 Authority US United States Prior art keywords cells cell gene regions dna Prior art date Legal status (The legal status is an assumption and is not a legal conclusion.

A Mechanism of Gene Amplification Driven by Small DNA Fragments Kuntal Mukherjee, Francesca Storici* School of Biology, Georgia Institute of Technology, Atlanta, Georgia, United States of America Abstract DNA amplification is a molecular process that increases the copy number of a chromosomal tract and often causes elevated.

Gene amplification and analysis vol.5, restriction endonucleases and methylases Edited by Jack G. Chirijian Elsevier Biomedical Press; Amsterdam, New York, x + pages. $, Dfl   96°C for 5 min to denature template and activate hot-start enzyme; five cycles of asymmetric amplification from LT6 T-DNA priming site (per cycle: 94°C for 10 s, 65°C for 30 s, 72°C for 2.

Abstract. Gene amplification is a ubiquitous process that occurs under conditions in which elevated levels of specific gene products provide a cell with a growth advantage or are required for cellular differentiation to proceed.

Gene amplification and analysis (OCoLC) Online version: Gene amplification and analysis (OCoLC) Material Type: Series: Document Type: Journal / Magazine / Newspaper: ISSN: OCLC Number: # Gene Amplification\/span>\n \u00A0\u00A0\u00A0\n schema.

Gene Amplification. Gene amplification means the abnormal presence of more than two copies of a gene in a nondividing cell (a few genes are normally present in more than one copy per diploid genome, e.g., α-globin). Gene amplification usually results in enhanced expression of the amplified gene and amplification of some proto-oncogenes is an important mechanism in the progression of some types.

We analyzed gene amplification mutants derived from parent strains in which the relocated catA gene was within 24 or 32 kbp of the nearest IS element (locus i or ii, respectively, in Fig.

As described here, duplication junction sequences were determined in order to identify IS involvement. What does amplification mean. amplification is defined by the lexicographers at Oxford Dictionaries as The process of increasing the volume of sound, especially using an amplifier.

Vol Issue 2. Article. Analysis of gene amplification and overexpression in human esophageal‐carcinoma cell lines. Yuji Kanda. Departments of Pathology, Faculty of Medicine, Kyoto University, Kyoto, Japan. Departments of Surgery, Faculty of Medicine, Kyoto University, Kyoto, Japan.

PCR, thermocyclers and some more software that works with Mac OS X. Author Summary DNA amplification is a copy-number increase of a DNA segment. Although DNA amplification is frequently observed in cancer and other degenerative disorders, the molecular mechanisms initiating this process are still largely elusive.

Here we demonstrate that small DNA fragments with homology to two distant loci on the same chromosomal arm can trigger amplification. VOLUME 3 Part 4: Gene Expression Analysis of Gene Regulation Using Reporter Systems Pradeep D.

Uchil, Arvindhan Nagarajan, and Priti Kumar RNA Interference and Small RNA Analysis Chengjian Li and Phillip D. Zamore Expressing Cloned Genes for Protein Production, Purification, and Analysis Clara L. Kielkopf, William Bauer, and Ina. Doris Mayr, MD, Veronika Kanitz, MD, Birgit Anderegg, PhD, Beate Luthardt, Jutta Engel, MD, Udo Löhrs, MD, Gudrun Amann, PhD, Joachim Diebold, MD, Analysis of Gene Amplification and Prognostic Markers in Ovarian Cancer Using Comparative Genomic Hybridization for Microarrays and Immunohistochemical Analysis for Tissue Microarrays, American Journal of Clinical Pathology, Volume.

Gene duplication (or chromosomal duplication or gene amplification) is a major mechanism through which new genetic material is generated during molecular can be defined as any duplication of a region of DNA that contains a duplications can arise as products of several types of errors in DNA replication and repair machinery as well as through fortuitous capture by selfish.A simple yeast TID amplification recovered after prolonged selection for gene amplification is diagrammed in Figure 9 (top).

One can account for its structure if the indicated palindromes (stems and loops) allowed a 3′ end to pair across a fork and prime a reverse-order copy as suggested (Araya et al.

; Brewer et al. ).Gene Amplification Subject Areas on Research A comprehensive survey of genomic alterations in gastric cancer reveals systematic patterns of molecular exclusivity and co-occurrence among distinct therapeutic targets.